| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mikulec, Isabella J (12)
|
NITWST |
31.33 |
30.69 |
-0.64 |
| 2 |
Mulawka, Paige M (12)
|
NIHAMB |
35.53 |
34.75 |
-0.78 |
| 3 |
Forcucci, Angela (12)
|
NITWST |
39.66 |
36.22 |
-3.44 |
| 4 |
Drozdowski, Jessica L (11)
|
NITWST |
42.85 |
36.62 |
-6.23 |
| 5 |
Barnes, Kelly R (12)
|
NITWST |
37.44 |
36.70 |
-0.74 |
| 6 |
Glogowski, Amelia C (11)
|
NITWST |
40.09 |
37.67 |
-2.42 |
| 7 |
Daley, Lily K (11)
|
NITWST |
40.53 |
37.88 |
-2.65 |
| 8 |
Latona, Dena M (12)
|
NIHAMB |
34.72 |
38.06 |
3.34 |
| 9 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
38.75 |
41.06 |
2.31 |
| 10 |
Astalos, Holly T (12)
|
NIHAMB |
46.03 |
41.17 |
-4.86 |
| 11 |
LeBaron, Julia L (11)
|
NIHHSC |
42.24 |
42.44 |
0.20 |
| 12 |
Blair, Gianna M (11)
|
NITTAC |
43.00 |
42.49 |
-0.51 |
| 13 |
Rudnicki, Meghan E (11)
|
NITWST |
46.20 |
42.91 |
-3.29 |
| 14 |
Hardy, Haleigha G (11)
|
NIPVSC |
47.54 |
44.11 |
-3.43 |
| 15 |
Mancuso, Gabriella J (11)
|
NIHAMB |
47.08 |
47.55 |
0.47 |
| 16 |
Jones, Sophia M (11)
|
NIPVSC |
47.15 |
47.69 |
0.54 |
| 17 |
McGowan, Jane S (12)
|
NIHAMB |
46.68 |
49.14 |
2.46 |
| 18 |
Regan, Maggie E (11)
|
NITWST |
56.17 |
53.56 |
-2.61 |
| 19 |
Wetherby, Breanne A (12)
|
NIEMAC |
|
1:18.20 |
- |
|
Gonzalez, Catherine E (12)
|
NIHAMB |
45.21 |
NS |
- |